Molecular Biology Toolkit

I. Solution Prep

Be careful with the units!

$$ Mass = Conc \times Vol \times MW $$

MW (g/mol)

Vol (L)

Conc (M)

The One Equation to Rule Them All

$$ V_{stock} = (C_{target} \times V_{total}) / C_{stock} $$

Stock (C₁)

Target (C₂)

Total Vol (V₂)

I know you do this a lot.

Sample (µg/µl)

Target Mass (µg)

1:1000 or 1:500? Just guess.

Total Volume (ml)

Ratio (1 : X)

Not for human consumption.

Acrylamide Target %

Total Gel Volume (ml)

Acrylamide Stock %

Tris Buffer Stock (M)

Tris Buffer Target (M)

SDS Stock %

SDS Target %

I'll admit this is pretty interesting for biology.

$$ Conc = A_{260} \times Factor \times Dilution $$

A₂₆₀

Type

Dilution Factor

It's probably contaminated.

A₂₆₀

A₂₈₀

A₂₃₀

Easy peasy lemon squeezy.

$$ \text{pmol} = \text{ng} \times 1000 / (\text{bp} \times 650) $$

Mass (ng)

Length (bp)